Ash dreamed of owning a Squirtle as his first Pokémon, but by the time he got to Professor Oak's Laborotory, Oak's grandson Gary Oak had already taken it. By the time of The Ties that Bind, it had evolved all the way into a Blastoise.
Ash captures his very own Squirtle, the leader of the Squirtle Squad. In his Johto journeys, he returns it to the Squirtle Squad, although it helps him out sometimes.
Team Go-Getters' SquirtleEdit
The main character in Pokémon Mystery Dungeon: Team Go-Getters out of the Gate! special is a Squirtle that claims that he used to be human. He joins Team Go-Getters in their rescue work. While he can't use Water Gun, he is happy to use Bubble because it looks pretty.
In The Right Place and the Right Mime, May befriended a young and timid Squirtle bellonging to Professor Oak and at the end of the episode, Oak gives Squirtle to her. During her Johto journeys, it evolves into a Wartortle.
In Journey to the Starting Line, a set of the three Kanto starter Pokémon in which a boy named Gilbert was supposed to choose from as his starting Pokémon were scared off by Ash's Tauros. Professor Oak, Tracey, Delia, and Gilbert had to split up and find the three missing Pokémon. Squirtle was the second one found. The Tiny Turtle Pokémon was stuck in a tree that was hanging over a cliff. Ash's Bulbasaur managed to catch the Squirtle before it could fall off the cliff.
Two Squirtle appeared in Battle Aboard the St. Anne. One was under the ownership of a Gentleman while the other was under the ownership of an unnamed Trainer. Squirtle also appeared in Pokémon Fashion Flash, The Breeding Center Secret and Dueling Heroes. A Squirtle under the ownership of an unknown Trainer, appeared in Friends to the End, during the closing ceremonies of the Indigo League. A Squirtle appeared in Marina's fantasy in Bye Bye Psyduck. A Squirtle was used by one of the students of the Pokémon Trainer's School in Gonna Rule the School!. Another Squirtle appeared in Sticking With Who You Know!, falling in love with a Raichu.