Dodrio first appeared in Pokémon - I Choose You!, where it has a brief appearance, while shouting its cry to the rising sun like a rooster, notably as the first Pokémon seen in real life in the anime (all Pokémon that had appeared beforehand in the episode were either on TV, in dreams, or not real).
Dodrio's first major appearance was in The Flame Pokémon-athon!. Dario used the three-headed bird to compete in a race, but was beaten by Ash who was riding Lara Laramie's Ponyta, after it evolved into Rapidash.
Candice used a Dodrio in A Pyramiding Rage!.
Dodrio served as mounts for the Diglett thieves in the episode, Plant It Now...Diglett Later!. A group of Dodrio were used in Pokémon Ranger and the Kidnapped Riolu! Part Two by Ash, Kellyn, Dawn and Brock to get to the Riolu that J had kidnapped. Dodrio also appeared in Primeape Goes Bananas and The March of the Exeggutor Squad. A Dodrio also appeared in Pokémon Fashion Flash. Dodrio also made a cameo in A Chansey Operation. This Dodrio had its necks tied in a knot. A Dodrio appeared in The Case of the K-9 Caper!. Dodrio made an appearance in Make Room for Gloom. Tracey was seen riding a Dodrio in a flashback in The Lost Lapras while Pokémon watching. However, it is unknown whether Dodrio belonged to him or if it was simply borrowed. Dodrio also appeared in The Power of One. Three Dodrio were seen at Professor Oak's Laboratory in A Tent Situation. Multiple Doduo appeared in the opening of Destiny Deoxys. A Dodrio made a cameo in Pokémon Ranger and the Temple of the Sea. A Dodrio appeared in Camping It Up!. A group of Dodrio also appeared in Historical Mystery Tour!.